Part:BBa_K1732000:Design

J23100-PelB-GlucHCO-B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 546
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 115
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This sequence is codon optimized for E.coli.

The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 3-4 uM of coelenterazine.

Sequence contains a PelB leader sequence which suggested that the Gaussia princeps luciferase proteins would be secreted into the expected location of the periplasmic space. Howeverm the observed location of the proteins was extracellular (in the media).

Source

Engineered from XXXXXXXX. Provided by the Bruchez lab at Carnegie Mellon.